Air oxidation of fluorene to fluorenone —separation of products by column chromatography chem 213 experiment 6 • synthesize the fluorene/fluoreneone mixture • separate the fluorene from fluorenone using the technique of column chromatography. A spot from each of the test tubes are added to a tlc plate as well as a spot of pure fluorene and fluorenone the solvent used in the column chromatography that was earlier determined, 10% ethyl acetate and hexanes, was also used as the solvent in the tlc chromatography.
Fluorenone remained in the column far longer than the fluorene, allowing all of the fluorene to be eluted while the visible yellow fluorenone band had hardly moved in.
Column chromatography: to pack the column, silica gel was mixed with 14ml of a non-polar solvent, hexane and transferred inside the column 1mm of sand was delivered to the column so that it would sit atop the silica gel bed to load the column, about 3ml of the mixture of fluorine and 9-fluorenone was delivered which was visible by a yellow band. Chromatographic separation of fluorene and fluorenone abstract: a mixture of fluorene (1) and fluorenone (2) was separated by column chromatography.
1 synthesis and chromatographic separation of fluorenone from flourene charlie page introduction chromatography has long been used in chemistry as a cheap and easy way to. Column chromatography will be used to separate fluorene from an oxiation product 9-fluorenone one of htese compounds is white and the other is yelloiww the progress of hte chromatography may be followed by evaporation of the solvent at periodic intervals as well as by observing teh slower-moving yellow band. 04 = fluorenone, 06 = fluorene flask three was only supposed to have fluorenone but this shows contamination of the other compound for a mixture of fluorene, fluorenone and -fluorenol is examined by tlc and gives the following rf values: 03, 05, 08| identify each spot and rationalize the relative rf values.
Column chromatography separation of fluorene and 9-fluorenone purpose: separate a mixture of fluorene and 9-fluorenone by column chromatography reading: palleros unit 9, column chromatography including the following: 1 column chromatography (page 197) 2 packing the column (page 197) 3 loading the sample (page 198) 4.
Column chromatography this is the last technique experiment in the introductory organic lab more importantly it is 1 predict the order of elution of fluorene and fluorenone from an alumina chromatography column, the polarity of the mobile phase as well as the rate of elution all affect the separation.